【摘要】The invention belongs to the technical field of experiment animals and medical image processing and application, and specifically relates to an evaluation method for tumour model effective intervention based on an optical imaging technology. The method comprises: using bioluminescence and fluorescent techniques to mark tumour model cells, using optical detection instruments to directly detect activities of the marked cells of a tumour model and behaviors of marked genes, observing expression or interaction of specific cells, genes, and molecules, and meanwhile detecting a plurality of kinds of molecular events, tracking targets, and imaging and evaluating an intervening measure effect from molecular and cellular level. The method can observe growth and metastasis of tumour cells in vivo and reaction after intervention in real time. Further, the method can be used for simply, conveniently, and accurately screen out effective intervention measures or effective intervention compatibility measures for different histological pattern tumours.