【摘要】The invention belongs to the field of cell biology and relates to a method for rapidly and fully obtaining funicle mesenchyme stem cells. The method comprises the following steps of: removing artery and vein of the funicle obtained under the sterile condition, preparing into tissue fragments, adding a phosphate buffer solution containing penicillin and streptomycin to clean, centrifuging to remove supernate, digesting the funicle fragments with collagenase NB4 and hyaluron, placing in a shaker in an ethylenediamine tetraacetate phosphate solution for oscillation, filtering with a filter screen of 100-200 meshes, collecting the filtrate, finally, centrifuging for 5-10 minutes with 600g, removing supernate, and cleaning for two to three times with DMEM (dulbecco's modified eagle medium) culture medium to obtain the funicle mesenchyme stem cells. In the invention, a large amount of funicle mesenchyme stem cells can be obtained in short time. Proved by experiments, the obtained funicle stem cells express the specific markers of the stem cells and ensure induction differentiation to fat, bone, cartilage, and the like.
