【摘要】The invention belongs to the field of cell biology and relates to a method for separating and purifying schwann cells. The method sequentially comprises the following steps of: firstly, taking a nerve segment under the aseptic condition and removing an epineurium secondly, digesting a nerve tract by using a NB4 composite collagenase and dispase mixed digestive enzyme solution and discarding the solution thirdly, placing the mixture in a trypsin and an ethylene diamine tetraacetic acid (EDTA) for 5 to 10 minutes to obtain a nerve fiber from which a perineurium is removed fourthly, placing the obtained nerve fiber in the mixed enzyme solution, and oscillating the mixture to obtain a cell suspension and finally, performing centrifugation and heavy suspension. By using the method, a great quantity of high-purity schwann cells are obtained in a low-cost and high-efficiency mode so that the abundant and high-quality schwann cells are supplied for repairing the damaged nerve.
