【摘要】The present invention, pertaining to the field of biomedicine and bioengineering, relates to the gene delivery system technology related to the gene therapy, specifically to a mediated gene conversion method. The invention combines the ultrasonic targeted damage microbubble technology and gland relevant viral vector technology at the appropriate time and mode to realize advantage complementary, so that the target gene can be more safely and efficiently entered in the histiocytes to realize the long-term stable expression of the target gene. The method described in this invention can be used in the vivo experimental investigation of AAV carrying of different target genes for gene therapy, specifically in the non-AAV direction cells or tissues the invention can safely improve the AAV carrying gene transfection efficiency, speed up gene body expression, increase the targeting of the transfection, reduce the virus dosage and diminish the toxic side effects.
