【卷号】FIRST PEOPLE S HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIVERSITY
【页码】CN102586175;|2012.07.18
【ISSN】CN2011104001353
【分类号】C12N5/074
【摘要】The invention belongs to the field of tissue engineering and relates to a method for culturing a human limbus stem cell graft by taking a biological amnion as a carrier. According to the method disclosed by the invention, the biological amnion is used as the carrier for culturing a limbus stem cell, wherein the biological amnion is a freeze-dried human amnion which is irradiated and sterilized by colbalt-60 the safety of the biological amnion is higher than that of denuded amniotic membrane and the biological amnion is easy to preserve and convenient to acquire and is widely used clinically. The method disclosed by the invention has the advantages that an acceptor can be prevented from being infected for a long term the preparation process is simple the pollution in the material obtaining and treating process can be reduced limbus epithelial cells can be cultured more safely and conveniently and the clinical application of the human limbus stem cell graft is promoted.
